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laemmli buffer  (Bio-Rad)


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    Structured Review

    Bio-Rad laemmli buffer
    Laemmli Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coomassie+brilliant+blue/Coomassie+Brilliant+Blue+G-250/pmc13141765-66-4-19
    Average 96 stars, based on 3626 article reviews
    laemmli buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Electrochemical biosensor based on cellulose nanocrystals functionalized with peptide-module probes for sensitive detection of influenza virus hemagglutinin.
    Article Snippet: The high transmissibility and rapid evolution of the influenza virus have contributed to its global spread.. Consequently, there is an urgent need for versatile and advanced approaches that enable rapid biosensor development for effective influenza virus detection and control.. In this study, we developed a sensitive electrochemical biosensor in which a fusion of cellulose-binding modules and virus-binding peptide (CBM31-VBP1) was site-specifically immobilized on a cellulose nanocrystal (CNC)-coated electrode.

    Staining:

    Article Title: Electrochemical biosensor based on cellulose nanocrystals functionalized with peptide-module probes for sensitive detection of influenza virus hemagglutinin.
    Article Snippet: The high transmissibility and rapid evolution of the influenza virus have contributed to its global spread.. Consequently, there is an urgent need for versatile and advanced approaches that enable rapid biosensor development for effective influenza virus detection and control.. In this study, we developed a sensitive electrochemical biosensor in which a fusion of cellulose-binding modules and virus-binding peptide (CBM31-VBP1) was site-specifically immobilized on a cellulose nanocrystal (CNC)-coated electrode.

    Article Title: Photosynthetic engineered living materials incorporating recombinant Chlamydomonas reinhardtii enable long-term semi-continuous photobiotransformation.
    Article Snippet: Signal development was done using Fuji Medical X-ray films (Fujifilm, Japan). .. After immunodetection membranes were stained with Coomassie Brilliant Blue (Bio-Rad, United Kingdom) to confirm equal protein load. ..

    Article Title: Nucleoside Modifications and Poly(A) Tail Length Greatly Influence Protein Expression from In Vitro-Transcribed mRNA in a Salmonid Cell Line
    Article Snippet: Duplicate samples of CHSE-214 cells transfected with mRNAs encoding EGFP/Lipo3000 or transfection reagent only were assessed using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and a Mini Protein Tetra cell (Bio-Rad Laboratories, Hercules, CA, USA). .. From the same gel, half of the gel (with one set of samples) was stained using Coomassie brilliant blue and the other half was subject to electroblotting and transferred to nitro-cellulose membranes (Bio-Rad) using a Bio-Rad mini transblot cell, and immunodetection was performed with rabbit-anti EGFP antibody (Chromotek, Planegg, Germany) diluted 1:1000. .. Secondary antibody, goat anti-rabbit conjugated with horseradish peroxidase (HRP) (Dako, Agilent Technologies, Glostrup, Denmark), was diluted 1:2000, developed using Clarity Western ECL Substrate (Bio-Rad), and pictures were taken with a GelDoc XR + Gel documentation system (BioRad).

    Article Title: RhlR quorum-sensing receptor ligand sensitivity regulates the differential expression of phenazine genes in Pseudomonas aeruginosa .
    Article Snippet: .. Gels were stained using Coomassie brilliant blue and imaged on a BioRad EZ-Doc gel imager. ..

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: .. Colonies and cells were evaluated after 24 h. For light microscopy, ~10 μL of culture was taken with a microbiological needle, suspended in sterile deionized water, air-dried, stained for 10–15 min with Coomassie Brilliant Blue (Bio-Rad Laboratories, Hercules, CA, USA), rinsed with Month XXXX Volume 0 Issue 0 10.1128/spectrum.02919-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 22 A pr il 20 26 b y 24 02 :8 00 :6 34 3: f2 77 :d c1 a: 6b 26 :c cc f: 9c 54 . distilled water, and observed at ×1,000 magnification for morphological examination using a Carl Zeiss Axio Imager two microscope (Jena, Germany). ..

    Article Title: Talin controls the spatial distribution of vinculin tension in focal adhesions
    Article Snippet: .. Gels were stained with Coomassie Brilliant Blue and imaged with a Bio-Rad ChemiDoc MP imaging system (590/110 white transmission). ..

    Article Title: CCL17 drives the expression of MMP9 and MMP13 expression via ERK1/2 and NF-κB signaling pathways in rheumatoid arthritis.
    Article Snippet: .. Gels were stained with Coomassie Brilliant Blue (BioRad) and destained to visualize proteolytic activity as clear bands against a blue background. .. Images were captured using the iBright imaging system (Thermo Fisher Scientific), and band intensity was quantified by densitometry.

    Bradford Assay:

    Article Title: Electrochemical biosensor based on cellulose nanocrystals functionalized with peptide-module probes for sensitive detection of influenza virus hemagglutinin.
    Article Snippet: The high transmissibility and rapid evolution of the influenza virus have contributed to its global spread.. Consequently, there is an urgent need for versatile and advanced approaches that enable rapid biosensor development for effective influenza virus detection and control.. In this study, we developed a sensitive electrochemical biosensor in which a fusion of cellulose-binding modules and virus-binding peptide (CBM31-VBP1) was site-specifically immobilized on a cellulose nanocrystal (CNC)-coated electrode.

    Immunodetection:

    Article Title: Photosynthetic engineered living materials incorporating recombinant Chlamydomonas reinhardtii enable long-term semi-continuous photobiotransformation.
    Article Snippet: Signal development was done using Fuji Medical X-ray films (Fujifilm, Japan). .. After immunodetection membranes were stained with Coomassie Brilliant Blue (Bio-Rad, United Kingdom) to confirm equal protein load. ..

    Article Title: Nucleoside Modifications and Poly(A) Tail Length Greatly Influence Protein Expression from In Vitro-Transcribed mRNA in a Salmonid Cell Line
    Article Snippet: Duplicate samples of CHSE-214 cells transfected with mRNAs encoding EGFP/Lipo3000 or transfection reagent only were assessed using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and a Mini Protein Tetra cell (Bio-Rad Laboratories, Hercules, CA, USA). .. From the same gel, half of the gel (with one set of samples) was stained using Coomassie brilliant blue and the other half was subject to electroblotting and transferred to nitro-cellulose membranes (Bio-Rad) using a Bio-Rad mini transblot cell, and immunodetection was performed with rabbit-anti EGFP antibody (Chromotek, Planegg, Germany) diluted 1:1000. .. Secondary antibody, goat anti-rabbit conjugated with horseradish peroxidase (HRP) (Dako, Agilent Technologies, Glostrup, Denmark), was diluted 1:2000, developed using Clarity Western ECL Substrate (Bio-Rad), and pictures were taken with a GelDoc XR + Gel documentation system (BioRad).

    Light Microscopy:

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: .. Colonies and cells were evaluated after 24 h. For light microscopy, ~10 μL of culture was taken with a microbiological needle, suspended in sterile deionized water, air-dried, stained for 10–15 min with Coomassie Brilliant Blue (Bio-Rad Laboratories, Hercules, CA, USA), rinsed with Month XXXX Volume 0 Issue 0 10.1128/spectrum.02919-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 22 A pr il 20 26 b y 24 02 :8 00 :6 34 3: f2 77 :d c1 a: 6b 26 :c cc f: 9c 54 . distilled water, and observed at ×1,000 magnification for morphological examination using a Carl Zeiss Axio Imager two microscope (Jena, Germany). ..

    Sterility:

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: .. Colonies and cells were evaluated after 24 h. For light microscopy, ~10 μL of culture was taken with a microbiological needle, suspended in sterile deionized water, air-dried, stained for 10–15 min with Coomassie Brilliant Blue (Bio-Rad Laboratories, Hercules, CA, USA), rinsed with Month XXXX Volume 0 Issue 0 10.1128/spectrum.02919-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 22 A pr il 20 26 b y 24 02 :8 00 :6 34 3: f2 77 :d c1 a: 6b 26 :c cc f: 9c 54 . distilled water, and observed at ×1,000 magnification for morphological examination using a Carl Zeiss Axio Imager two microscope (Jena, Germany). ..

    Microscopy:

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: .. Colonies and cells were evaluated after 24 h. For light microscopy, ~10 μL of culture was taken with a microbiological needle, suspended in sterile deionized water, air-dried, stained for 10–15 min with Coomassie Brilliant Blue (Bio-Rad Laboratories, Hercules, CA, USA), rinsed with Month XXXX Volume 0 Issue 0 10.1128/spectrum.02919-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 22 A pr il 20 26 b y 24 02 :8 00 :6 34 3: f2 77 :d c1 a: 6b 26 :c cc f: 9c 54 . distilled water, and observed at ×1,000 magnification for morphological examination using a Carl Zeiss Axio Imager two microscope (Jena, Germany). ..

    Imaging:

    Article Title: Talin controls the spatial distribution of vinculin tension in focal adhesions
    Article Snippet: .. Gels were stained with Coomassie Brilliant Blue and imaged with a Bio-Rad ChemiDoc MP imaging system (590/110 white transmission). ..

    Transmission Assay:

    Article Title: Talin controls the spatial distribution of vinculin tension in focal adhesions
    Article Snippet: .. Gels were stained with Coomassie Brilliant Blue and imaged with a Bio-Rad ChemiDoc MP imaging system (590/110 white transmission). ..

    Activity Assay:

    Article Title: CCL17 drives the expression of MMP9 and MMP13 expression via ERK1/2 and NF-κB signaling pathways in rheumatoid arthritis.
    Article Snippet: .. Gels were stained with Coomassie Brilliant Blue (BioRad) and destained to visualize proteolytic activity as clear bands against a blue background. .. Images were captured using the iBright imaging system (Thermo Fisher Scientific), and band intensity was quantified by densitometry.



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